Gene trap insertional mutagenesis in mice: New vectors and germ Line mutations in two novel genes |
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Authors: | Edward G. Neilan Gregory S. Barsh |
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Affiliation: | (1) Departments of Pediatrics, Genetics, Developmental Biology, USA;(2) Howard Hughes Medical Institute, Stanford University, Palo Alto, CA 94305, USA |
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Abstract: | Insertional mutagenesis based on gene trap vectors that capture endogenous splice sites is a promising tool for functional genomics. Several groups have proposed large-scale gene trap screens, but questions remain as to the type of vectors and their design. We report a set of plasmid-encoded gene trap vectors and the disruption of two novel genes. Our results include a comparison of the relative gene trapping efficiencies of two different splice acceptor sequences in ES cells and an analysis of the structure of several gene trap insertions. |
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Keywords: | gene trap embryonic stem cells electroporation vectors |
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