Genotypic differences and alterations of protein patterns of tomato explants under copper stress

In vitro response of six tomato genotypes to different copper concentrations was studied. Cu was toxic to tomato explants at a relatively high concentration (100 μM), which reduced callus growth and shoot regeneration. Peto-86 followed by UC-97-3 were more tolerant to copper than the other genotypes. Cu (100 μM) induced the synthesis of eight new proteins (70.86 - 14.78 kD) in Peto-86 and six in Western Improve (46.43 - 14.78 kD) and UC-97-3 (77.69 - 14.78 kD). Cu-stress reduced the expression of some enzymatic bands of alcohol dehydrogenase and esterase, meanwhile, one peroxidase band at the locus Prx-1 was newly expressed under Cu-treatment. This revised version was published online in July 2006 with corrections to the Cover Date.