| 荧光漂白后恢复技术及其在活细胞分子机制研究中的应用 |
| |
| 引用本文： | 张志毅,周涛,巩伟丽,张德添.荧光漂白后恢复技术及其在活细胞分子机制研究中的应用[J].生物技术通讯,2008,19(4):635-637. |
| |
| 作者姓名： | 张志毅 周涛 巩伟丽 张德添 |
| |
| 作者单位： | 国家生物医学分析中心,北京,100850 |
| |
| 摘 要： | 荧光漂白后恢复（FRAP）是一项利用荧光探针研究活体细胞中各类分子迁移特性的技术。简要介绍了FRAP技术的原理和具体实施要求,列出了动态比和扩散系数的计算公式,并例举了近几年FRAP技术在细胞分子机制研究中的应用。
|
| 关 键 词： | 荧光漂白后恢复 荧光漂白损失 动态比 扩散系数 |
Fluorescence Recovery After Photobleaching and its Applications in the Study of Cellular Molecular Mechanics |
| |
| ZHANG Zhi-yi,ZHOU Tao,GONG Wei-li,ZHANG De-tian.Fluorescence Recovery After Photobleaching and its Applications in the Study of Cellular Molecular Mechanics[J].Letters in Biotechnology,2008,19(4):635-637. |
| |
| Authors: | ZHANG Zhi-yi ZHOU Tao GONG Wei-li ZHANG De-tian |
| |
| Institution: | (National Center of Biomedical Analysis,Beijing 100850,China ) |
| |
| Abstract: | Fluorescence recovery after photobleaching（FRAP） is a technology for studying the mobility of molecules tagged with fluorophores in living cells.In this paper,the principle and operating request of FRAP were introduced,the formulas of mobile fraction and diffusion coefficien presentedt,and finally some cases of the FRAP applications in the study of cellular molecular mechanics in recent years were gived. |
| |
| Keywords: | fluorescence recovery after photobleaching fluorescence loss in photobleaching mobile fraction diffusion coefficient |
| 本文献已被 维普 万方数据 等数据库收录！ |
|
