Production of recombinant hirudin in Hansenula polymorpha: variation of gene expression level depends on methanol oxidase and fermentation strategies |
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Authors: | C H Kim H W Seo H Y Kim J H Sohn E S Choi S K Rhee |
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Affiliation: | (1) Applied Microbiology Research Division, Korea Research Institute of Bioscience and Biotechnology, PO Box 115, Yusong, Taejon 305–600, Korea, KR |
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Abstract: | Various recombinant Hansenula polymorpha strains were developed and compared for their level of expression of the anticoagulant hirudin. H. polymorpha DL1-57 harboring an autonomously replicating sequence, HARS36, efficiently expressed the gene for recombinant hirudin. The effect of methanol oxidase (MOX) on the expression of the hirudin gene in H. polymorpha DL1-57 was studied, and the fermentation strategies coupled with the MOX activity and an antioxidant, tocopherol, were also examined. Received 4 February 1998/ Accepted in revised form 24 June 1998 |
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Keywords: | : hirudin Hansenula polymorpha methylotrophic yeast methanol oxidase autonomously replicating sequence |
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