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| 12个水牛群体催乳素基因第4外显子遗传特征分析 | |
| 作者姓名： | Yuan F Miao YW Li DL Tang SK Xv Z Huo JL Qi H |
| 作者单位： | 1. 云南农业大学,动物科学技术学院,云南,昆明,650201 2. 云南农业大学,动物科学技术学院,云南,昆明,650201;云南大学,云南省生物资源保护与利用重点实验室,云南,昆明,650091 3. 云南水牛科学技术研究所,云南,昆明,650021 4. 云南省德宏州潞西市畜牧站,云南,潞西,678400 5. 广西畜牧总站,广西,南宁,530022 |
| 基金项目： | 云南省应用基础研究重点项目(2007C0003Z);云南省应用基础研究计划面上项目(2010ZC243);云南省应用基础研究计划面上项目(2006C0034M); 国家自然科学基金项目(30660024); 国家高技术研究发展计划(“863”计划)项目(2008AA101001); 云南省教育厅科学研究基金项目(2010Y344) |
| 摘 要： | 催乳素对水牛乳腺发育、泌乳和乳蛋白基因的表达有明显的调控作用。该研究采用直接测序并结合PCR-SSCP技术,分析沼泽型水牛和河流型水牛12个群体385个个体的催乳素基因(PRL)第4外显子(exon4)的遗传特征。结果表明:水牛PRLexon4由180个核苷酸组成,在不同物种中具有高度保守性。序列分析发现水牛中该外显子的第109位碱基处有C>T替换,为沉默突变,与泌乳性状之间无显著相关性。在9个沼泽型水牛群体中均检测到该突变位点,其中PBA基因在7个沼泽型水牛群体中为优势基因,PBB基因在德宏和富钟群体中为优势基因,沼泽型水牛群体中PBA基因频率在0.400～0.917之间,群体平均值为0.629±0.049,具有地域分布特征。在3个河流型水牛群体中,第109位核苷酸处均为C,未检测到多态。提示河流型水牛与沼泽型水牛已有较大的遗传分化。 |
| 关 键 词： | 河流型水牛 沼泽型水牛 催乳素 Exon4 测序 PCR-SSCP |
Genetic characteristics on exon 4 of prolactin gene in 12 water buffalo populations |
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| Yuan F,Miao YW,Li DL,Tang SK,Xv Z,Huo JL,Qi H.Genetic characteristics on exon 4 of prolactin gene in 12 water buffalo populations[J].Zoological Research,2010,31(6):575-580. | |
| Authors: | Yuan Feng Miao Yong-Wang Li Da-Lin Tang Shou-Kun Xv Zheng Huo Jin-Long Qi Hong |
| Institution: | YUAN Feng1,MIAO Yong-Wang1,2,*,LI Da-Lin3,TANG Shou-Kun4,Xü Zheng5,HUO Jin-Long1,QI Hong1 (1. Faculty of Animal Science and Technology,Yunnan Agricultural University,Kunming 650201,China,2. Laboratory for Conservation and Utilization of Bio-resources,Yunnan University,Kunming 650091,3. Yunnan Institute of Buffalo Science and Technology,Kunming 650021,4. Animal Husbandry and Veterinary Station of Luxi City,Luxi 678400,5. Animal Husbandry Station of Guangxi Province,Nan... |
| Abstract: | The prolactin exerts obvious adjustment and control function for mammary gland development, lactation and milk protein gene expression in water buffalo. In this study the sequence features and polymorphisms of the exon 4 in prolactin gene were examined in 385 individuals which came from 12 river and swamp type buffalo populations using DNA direct sequencing and PCR-SSCP methods. The results showed that the sequence of exon 4 in prolactin gene was consists of 180 nucleotides, the fragment had high conservati... |
| Keywords: | River buffalo Swamp buffalo Prolactin Exon 4 Se quencing PCR-SSCP |
| 本文献已被 CNKI 万方数据 PubMed 等数据库收录！ | |