Effect of carbon source and pH of the growth medium on spore germination inPhycomyces blakesleeanus |
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Authors: | Guadalupe Martínez-Cadena Jorge Saavedra-Calixto Griselda Messina-Valencia Graciela Domínguez-Gutiérrez Guadalupe Novoa-Martínez |
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Institution: | (1) Instituto de Investigación en Biología Experimental, Facultad de Química, Universidad de Guanajuato, Apartado Postal 187, 36000 Guanajuato, Gto., Mexico |
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Abstract: | Phycomyces blakesleeanus sporangiospores responded differently to activation by physical and chemical stimuli. Spores that were physically (heat shock)
activated or chemically (ammonium acetate) activated germinated and grew at pH 4.5 with the hexoses glucose, fructose, galactose,
andN-acetylglucosamine, and with glycerol and amino acids. Under these conditions, physically activated spores showed a lower,
although significant growth with the hexoses fructose, galactose,N-acetylglucosamine and with glycerol. On the other hand, physically activated spores incubated at alkaline pH (pH 7.3) required
glucose to germinate; a requirement not observed with chemically activated spores, which showed significant growth in the
other hexoses tested. Both physically and chemically activated spores incubated at pH 7.3 were unable to germinate and grow
with amino acids and glycerol. These results suggest that there are different targets for activation of the spores by physical
and chemical treatments. The levels of the fermentative enzymes alcohol dehydrogenase and lactate dehydrogenase and of the
oxidative enzyme NAD+-isocitrate dehydrogenase were higher in cells grown at pH 4.5 in medium containing glucose; however, alcohol dehydrogenase
and lactate dehydrogenase appear not to be affected by a change in the pH of the growth medium. |
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Keywords: | Spore activation Phycomyces Carbon source pH |
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