Molecular and catalytic properties of 2,4-diacetylphloroglucinol hydrolase (PhlG) from Pseudomonas sp. YGJ3 |
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| Authors: | Hiroki Saitou Manami Watanabe Kiyofumi Maruyama |
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| Institution: | Department of Biomolecular Science, Faculty of Engineering, Gifu University, Gifu, Japan. |
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| Abstract: | Gene phlG encoding 2,4-diacetylphloroglucinol hydrolase was cloned from Pseudomonas sp. YGJ3 and expressed in Escherichia coli. Recombinant PhlG was purified homogeneously. It required 2-mercaptoethanol for stability. Km for 2,4-diacetylphloroglucinol and kcat were determined to be 24 μM and 5.8 s(-1) respectively. CoCl2 specifically and significantly activated PhlG. |
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