Scanning electron microscopy of freeze-fractured sclerotia of Physarum polycephalum |
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Authors: | N Kislev I Chet |
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Institution: | Department of Plant-Pathology and Microbiology, The Hebrew University, Faculty of Agriculture, Rehovot, Israel |
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Abstract: | The ultrastructure and architecture of freeze-fractured sclerotia of Physarum polycephalum was studied by a scanning electron microscope (SEM). The sclerotia are built of many spherules grouped together in a common outer coat. Each spherule has hard walls which separate it from its neighbors. The spherules are rounded in 2-day-old sclerotia, and have a lobe-like structure 3 weeks later. A new simple technique for obtaining freeze-fractured biological material is described and compared with freeze-fracture in a freeze-etching apparatus. The ultrastructural details of the fractured sclerotia are described. |
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