Cleavage specificity of boar acrosin on polypeptide substrates, ribonuclease and insulin B-chain. |
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Authors: | H Schiessler W D Schleuning H Fritz |
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Abstract: | The cleavage specificity of boar acrosin is, like that of trypsin, strictly limited to the arginyl and lysyl bonds, as demonstrated for the oxidized B-chain of insulin. In addition, in this polypeptide substrate as well as in reduced and carboxymethylated ribonuclease, these peptide bonds are hydrolyzed by acrosin and trypsin with nearly identical velocities. |
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