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O-glycan profiling of serum glycan for potential renal cancer biomarkers |
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| Authors: | Yi Gao Fei Ma Wei Zhang Fan Zhong HaiLin Tang DanFeng Xu Liang Zhao |
| Institution: | 1. Department of Urology, Changzheng Hospital of Second Military Medical University, Shanghai, 200003, China 2. State Key Laboratory of Proteomics, Beijing Proteome Research Center, Beijing Institute of Radiation Medicine, Beijing, 102206, China 3. Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai, 200237, China 4. Department of Automatic Control, College of Mechatronics and Automation, National University of Defense Technology, Changsha, 410073, China 5. Laboratory of Systems Biology, Institutes of Biomedical Sciences, Fudan University, Shanghai, 200433, China |
| Abstract: | Serum was obtained from 25 male renal cell carcinoma (RCC) patients and 21 healthy males. O-glycans were released by a β-elimination reaction and purified by graphitized carbon cartridge solid phase extraction, then profiled by matrix-assisted laser desorption/ionisation-time of flight mass spectrometry. After noise removal and peak alignment, 1372 peaks were extracted from 200000 data points. Feature peaks were analyzed by calculation of differential sensitivity and specificity. The combination of two feature peaks was chosen as a biomarker and could clearly differentiate RCC and normal samples in our study group. |
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