Cloning and sequencing of the cDNA encoding the pituitary gonadotropin II β-subunit of yellowfin porgy (Acanthopagrus latus) |
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Authors: | Huai-Jen Tsai Liang-Tung Yang |
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Affiliation: | Institute of Fisheries Science, National Taiwan University, Taipei, Taiwan 106 |
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Abstract: | The complementary DNA (cDNA) encoding the gonadotropic hormone (GTH) II pre-β-subunit of yellowfin porgy ( Acanthopagrus latus ) was isolated from a pituitary gland cDNA phage library. The cDNA insert, 598 base pairs (bp), contained a 411 bp open reading frame with 35 bp and 152 bp flanking regions at the 5'- and 3'-ends, respectively. The deduced amino acid sequence revealed a putative signal peptide of 24 amino acid residues and a 113 amino acid mature β-subunit of GTH polypeptide. This pre-β-subunit polypeptide of the yellowfin porgy GTH II showed 86% sequence identity with that of bonito GTH II-β 73% with killifish GTH II-β, 63% with African catfish GTH II-β 61% with pike eel GTH-β, 60% with silver carp GTH-β, 59% with chum salmon GTH 11-β, 58% with common carp GTH-β 57% with chinook Pacific salmon GTH-β, and 53% with European eel GTH II-β. |
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Keywords: | marine fish pituitary glycoprotein gonadotropin β-subunit complementary DNA sequence identity molecular phylogenetic tree |
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