An amperometric cellobiose dehydrogenase-based biosensor can be used for measurement of cellulase activity |
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| Authors: | Hildén L Eng L Johansson G Lindqvist S E Pettersson G |
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| Institution: | Department of Biochemistry, Uppsala University, SE-751 23 Uppsala, Sweden. lars.hilden@biokem.uu.se |
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| Abstract: | The hemoflavoenzyme cellobiose dehydrogenase (CDH, EC 1.1.99.18) from Phanerochaete chrysosporium has been used in an amperometric redox polymer-based biosensor. Used in conjugation with a FIA system this biosensor can replace colorimetric assays for measuring cellobiose liberated from cellulose in a series of cellulase-containing samples. The biosensor gave the same result as the Somogyi-Nelson method in a less time-consuming and laborious manner. The two methods showed about the same precision. |
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