Individualization and estimation of relatedness in crocodilians by DNA fingerprinting with a Bkm-derived probe |
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Authors: | J W Lang R K Aggarwal K C Majumdar and L Singh |
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Institution: | (1) Department of Biology, University of North Dakota, Grand Forks, USA;(2) Centre for Cellular and Molecular Biology, 500 007 Hyderabad, India |
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Abstract: | Individual-specific DNA fingerprints of crocodilians were obtained by the use of Bkm-2(8) probe. Pedigree analyses of Crocodylus palustris, C. porosus and Caiman crocodilus revealed that the multiple bands (22–23 bands with Aludigest) thus obtained were inherited stably in a Mendelian fashion. Unique fingerprints permitted us to identify individuals, assign parentage, and reconstruct the DNA profile of a missing parent. Average band sharing between unrelated crocodiles was found to be 0.37. Band sharing between animals of known pedigrees increased predictably with relatedness and provided a basis for distinguishing relatives from non-relatives. Similar results obtained in other species/genera, using the same probe, suggest that this approach may be applicable to all species of crocodilians, and could facilitate genetic studies of wild and captive populations. |
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Keywords: | Bkm DNA Genetic/DNA fingerprinting Crocodilians Paternity determination Band sharing |
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