Development of the entomopathogenic fungus Beauveria bassiana in liquid cultures |
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Authors: | Michael J. Bidochka Tom A. Pfeifer George G. Khachatourians |
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Affiliation: | (1) Bioinsecticide Laboratory, Dept. of Applied Microbiology and Food Science, University of Saskatchewan, S7N 0W0 Saskatoon, Sask, Canada |
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Abstract: | Growth and development of B. bassiana was followed in four liquid media: peptone, peptone-glucose, glucose and glucose-peptone-yeast extract. Six developmental stages were defined: (I) the unswollen conidium, (II) the swollen conidium, (III) emergence of the germ tube, (IV) elongation of the germ tube and formation of the first septum, (V) polar and bipolar elongation (growth) of the resulting mycelium and initiation of a blastospore and, (VI) seccession of that blastospore. Conidia of B. bassiana produced germ tubes in all liquid media. Blastospores were produced in all liquid media except glucose. In peptone-glucose, the yield of blastospores was four-fold higher than in glucose-peptone-yeast extract. However, biomass production was highest in peptone-glucose-yeast extract. |
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Keywords: | Beauveria bassiana cytodifferentiation blastospore production |
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