A new approach to the synthesis of APTRA indicators |
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Authors: | Otten P A London R E Levy L A |
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Institution: | National Institute of Environmental Health Sciences, Laboratory of Structural Biology, P.O. Box 12233, MR-01, Research Triangle Park, North Carolina 27709, USA. |
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Abstract: | We have devised a general synthesis of Mg(2+) indicators which is based on the aminophenol triacetic acid (APTRA) structure. The key step is a palladium-catalyzed coupling reaction of a precursor of the APTRA ligand with a fluorescent group. This strategy resulted in new ratioable fluorescent APTRA indicators and the finding that the fluorescence response of these indicators is different for Mg(2+) and Ca(2+) in some cases. We believe that this represents a generally useful approach for combining fluorophore and chelator functionalities. |
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