Novel microsatellite loci in the threatened European long-snouted seahorse (<Emphasis Type="Italic">Hippocampus guttulatus</Emphasis>) for genetic diversity and parentage analysis |
| |
Authors: | Belén G Pardo Almudena López Paulino Martínez Carmen Bouza |
| |
Institution: | (1) Departamento de Xenética, Facultade de Veterinaria, Universidade de Santiago de Compostela, Campus de Lugo, 27002, Lugo, Spain |
| |
Abstract: | The long-snouted seahorse Hippocampus guttulatus is one of the two European seahorse species. We describe the isolation of the first 12 microsatellite loci in this threatened
species. These new markers were tested in non-invasive samples of 32 seahorses from NW Spain. The number of alleles ranged
from 2 to 15 (mean: 6.3) and expected heterozygosity from 0.031 to 0.912 (mean: 0.500). All loci conformed to Hardy–Weinberg
expectations and no genotypic disequilibrium was observed between any pair of loci. The theoretical exclusion probabilities
for this set of loci, when no parental information exists or when one parent is known, were 0.973 and 0.998, respectively.
This study indicates the usefulness of these novel loci for population analysis and kinship studies in Hippocampus guttulatus. Their potential application is extended to the other European seahorse species, since all loci were successfully cross-amplified
in H. hippocampus. |
| |
Keywords: | Seahorse Hippocampus guttulatus Microsatellites Enriched library Polymorphism Parentage |
本文献已被 SpringerLink 等数据库收录! |
|