核壳结构CdS-TiO_2纳米颗粒体外光催化灭活白血病HL60细胞实验研究 |
| |
引用本文: | 张卿,罗有焕,鲁丽,陈丽,艾保全,熊建文. 核壳结构CdS-TiO_2纳米颗粒体外光催化灭活白血病HL60细胞实验研究[J]. 激光生物学报, 2016, 0(3): 214-222. DOI: 10.3969/j.issn.1007-7146.2016.03.005 |
| |
作者姓名: | 张卿 罗有焕 鲁丽 陈丽 艾保全 熊建文 |
| |
作者单位: | 1. 华南师范大学物理与电信工程学院,广东 广州,510006;2. 广东工业大学物理与光电工程学院,广东 广州,510006 |
| |
基金项目: | 国家自然科学基金(61072029);广州市科技计划资助项目(2014J4100049) |
| |
摘 要: | 本文研究了核壳结构CdS-TiO_2纳米颗粒光对HL60细胞光催化灭活作用,并初步探讨了CdS量子点增强TiO_2光催化灭活效率的作用机理。实验利用超声法制备了核壳结构CdS-TiO_2纳米颗粒,使用CCK-8法检测了其对白血病HL60细胞的光催化灭活效果,并采用活性氧分析和荧光发光光谱等分析手段对CdS量子点增强TiO_2光催化灭活效率的作用机理进行了分析。细胞实验结果表明,在暗室条件下,随着CdS包裹的TiO_2外壳的增厚,细胞的暗室存活率从28.5%逐渐上升至80%;在可见光照下,细胞的存活率显著下降。CdS-TiO_2纳米颗粒对HL60细胞的光催化灭活率均超过60%,其中CdS-TiO_2样品0.6对HL60细胞的光催化灭活效率最高,达到95%。根据荧光光谱和活性氧含量分析的结果推测,这可能是由于核壳结构的CdS内核与TiO_2外壳之间产生了有效的电子转移,抑制了TiO_2的空穴电子的复合,增强了TiO_2外壳的光催化活性,最终增加了TiO_2对HL60细胞的PDT灭活效果。
|
关 键 词: | CdS-TiO2 纳米颗粒 光动力疗法(PDT) 白血病 HL60 细胞 |
Photocatalytic Inactivation of Core-Shell CdS-TiO2 Nanoparticles on Leukemia HL60 Cancer Cells |
| |
Abstract: | The photocatalytic inactivation of core-shell cadmium sulfide-titanium dioxide nanoparticles (CdS-TiO2 nano-particles)on HL60 cells and its mechanism were researched in this paper.Core-shell cadmium sulfide-titanium dioxide (CdS-TiO2 )nanoparticles were synthesized by ultrasonic method.The photocatalytic inactivation of these core-shell nan-oparticles on HL60 cells were analyzed by CCK-8 assay.Its mechanism were investigated using reactive oxygen species (ROS)generation and fluorescence emission spectrum analysis.Results of cell experiment showed that,in comparison with the cell group of CdS,viability of cells treated with CdS-TiO2 increased from 28.5% to 80%,with the increment of TiO2 shell thickness in the darkroom conditions.However,under light exposure,their viability decreased sharply.More than 60% cells were killed in each group treated by CdS-TiO2 .Among these samples,cell groups treated by CdS-TiO2 sample 0.6 showed the highest cell inactivated efficiency of 95%.According to the results of ROS generation and FS a-nalysis,it could be concluded that the existence of CdS resisted the recombination of photo-induce holes and electrons, enhancing the photocatalytic activity of TiO2 shell and eventually resulting in superior cell killing effect. |
| |
Keywords: | TiO2 nanoparticles photodynamic therapy leukemia HL60 cells |
本文献已被 CNKI 万方数据 等数据库收录! |
|