MICB基因多态性与乳腺癌的易感关联性研究

目的:研究海南汉族人群MICB等位基因的多态性与乳腺癌易感性之间的关联性。方法:采用PCRSSP(PCR sequence-specific primers)和PCR-SBT(PCR sequence-based typing)方法对样本MICB等位基因的多态性进行检测。结果:乳腺癌患者中检出14种MICB等位基因;和对照组相比较,MICB*002和MICB*014等位基因在乳腺癌患者组分布频率较少,MICB*002和MICB*014等位基因可能对乳腺癌不易感(MICB*002:OR=0.31,95%CI:0.19-0.51,Pc0.05;MICB*014:OR=0.32,95%CI:0.17-0.60,Pc0.05)。MICB*016和MICB*003等位基因在乳腺癌患者组分布较多;MICB*016和MICB*003等位基因可能对乳腺癌易感(MICB*016:OR=10.68,95%CI:2.52-45.28,Pc0.05;MICB*003:OR=3.57,95%CI:1.34-9.49,Pc0.05);MICB*002/002和MICB*014/014基因型可能对乳腺癌不易感(MICB*002/002:OR=0.12,95%CI:0.04-0.36,Pc0.05;MICB*014/014:OR=0.30,95%CI:0.10-0.89,Pc0.05)。结论:MICB等位基因的多态性与乳腺癌的易感性之间存在关联性。

A Study on the Association between Polymorphism of MICB Gene and Breast Cancer

Objective:To study the association MICB gene polymorphism and breast cancer in han nationality of Hain-an.Methods:PCR-SSP and PCR-SBT were used to analyze the gene polymorphism of MICB.Results:Forty allele genes were found in patients of breast cancer,The frequencies of MICB*002 and MICB*014 were lower in breast cancer pa-tients than in healthy controls,although the frequencies of MICB*016 and MICB*003 were higher;MICB*016 and MICB*003 allele genes may be susceptible to breast cancer (MICB *016:OR=10.68,95% CI:2.52-45.28,Pc<0.05;MICB*003:OR=3.57,95% CI:1.34-9.49,Pc<0.05),and MICB*002 and MICB*014 allele genes may-be protected(MICB*002:OR=0.31,95% CI:0.19-0.51,Pc<0.05;MICB*014:OR=0.32,95% CI:0.17-0.60,Pc<0.05);MICB* 002/002 and MICB*014/014 genotypes maybe protected to breast cancer (MICB*002/002:OR=0.12,95% CI:0.04-0.36,Pc<0.05;MICB*014/014:OR=0.30,95% CI:0.10-0.89,Pc<0.05).Conclu-sions:MICB allele gene polymorphism may be associated with breast cancer.