Isolation of a subfraction of rough endoplasmic reticulum closely associated with mitochondria : Evidence for its role in cytochrome P450 synthesis |
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| Authors: | P. J. Meier M. A. Spycher U. A. Meyer |
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| Affiliation: | Division of Clinical Pharmacology, Department of Medicine, University of Zürich, Kantonsspital, 8091 Zürich, Switzerland |
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| Abstract: | A subfraction of rough endoplasmic reticulum (RER) structurally associated with mitochondria (mito-RER complexes) was isolated from crude nuclear fractions of rat liver homogenate. When apocytochrome P450 synthesis (which presumably occurs in RER) and mitochondrial heme synthesis was dissociated by concomitant treatment of rats with phenobarbital and cobaltous chloride, apocytochrome P450 accumulated predominantly in mito-RER complexes. These data suggest that cytochrome P450 synthesis requires structural interaction of mitochondria and RER. |
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