| 去乙酰化酶1基因对牛前体脂肪细胞凋亡影响的研究 |
| |
| 引用本文： | 刘晓牧,宋恩亮,刘桂芬,吴公莹,谭秀文,刘振山,万发春.去乙酰化酶1基因对牛前体脂肪细胞凋亡影响的研究[J].生物化学与生物物理进展,2010,37(3):297-303. |
| |
| 作者姓名： | 刘晓牧 宋恩亮 刘桂芬 吴公莹 谭秀文 刘振山 万发春 |
| |
| 作者单位： | 山东省农业科学院畜牧兽医研究所;山东省畜禽疫病防治与繁育重点实验室;山东畜牧兽医职业学院; |
| |
| 基金项目： | 国家高技术研究发展计划(863)(2008AA101010); 国家现代农业(奶业)产业技术体系资助项目(nycytx-02-04)~~ |
| |
| 摘 要： | 去乙酰化酶1(sirtuin type1,SIRTl)是一个新的脂肪细胞调控因子,通过与其靶基因叉头转录因子1(the forkhead box O family1,FoxO1)相互作用,参与细胞增殖、分化、衰老、凋亡和代谢过程.利用吖啶橙(acridine orange,AO)染色、流式细胞仪、荧光定量PCR(quantitative real-timePCR,qPCR)等技术方法,研究SIRT1的抑制剂———烟酸胺(nicotinamide,NAM)处理后对鲁西黄牛皮下前体脂肪细胞(bovine subcutaneous preadipocytes,BSP)和肌内前体脂肪细胞(bovine intramuscular preadipocytes,BIP)凋亡的影响.观察了BSP和BIP的凋亡形态;比较了SIRT1基因抑制后,相关基因如FoxO1等在两种细胞之间的表达差异.结果表明,NAM对BSP和BIP细胞表现出相同的生长抑制作用,处理组的BSP和BIP细胞的凋亡率均显著高于对照组,其作用可能通过抑制SIRT1,激活FoxO1凋亡通路实现.SIRT1及其相关基因对BSP和BIP的调控存在不同途径.
|
| 关 键 词： | 去乙酰化酶1(SIRT1) 烟酰胺 牛 前体脂肪细胞 凋亡 |
| 收稿时间： | 2009/10/8 0:00:00 |
| 修稿时间： | 2009/12/21 0:00:00 |
Effect of SIRT1 on Apoptosis of Bovine Preadipocytes |
| |
| LIU Xiao-Mu,SONG En-Liang,LIU Gui-Fen,WU Gong-Ying,TAN Xiu-Wen,LIU Zhen-Shan and WAN Fa-Chun.Effect of SIRT1 on Apoptosis of Bovine Preadipocytes[J].Progress In Biochemistry and Biophysics,2010,37(3):297-303. |
| |
| Authors: | LIU Xiao-Mu SONG En-Liang LIU Gui-Fen WU Gong-Ying TAN Xiu-Wen LIU Zhen-Shan and WAN Fa-Chun |
| |
| Institution: | LIU Xiao-Mu1,2),SONG En-Liang1,LIU Gui-Fen1,WU Gong-Ying3),TAN Xiu-Wen1,LIU Zhen-Shan1,WAN Fa-Chun1,2)** (1) Institute of Animal Science , Veterinary Medicine,Sh,ong Academy of Agricultural Sciences,Jinan 250100,China,2) Sh,ong Key Laboratory of Animal Disease Control , Breeding,3) Sh,ong Vocational Animal Science , Veterinary College,Weifang 261061,China) |
| |
| Abstract: | Sirtuin type 1 (SIRTl), a novel regulatory factor of adipocyte and myocyte, interacts with target the forkhead box O family 1 (FoxOl) to modulate cell proliferation and differentiation, aging, apotosis and metabolism. The effect of nicotinamide (NAM, inhibitor of SIRT1) on the apoptosis of bovine preadipocytes was investigated with Acridine Orange (AO) staining, the flow cytometry detection and quantitative real-time PCR (qPCR). The results showed that NAM inhibited the differentiation of bovine subcutaneou... |
| |
| Keywords: | SIRT1 nicotinamide bovine preadipocytes apoptosis |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《生物化学与生物物理进展》浏览原始摘要信息 |
| 点击此处可从《生物化学与生物物理进展》下载免费的PDF全文 |
|
