Mastocytoma-medicated suppression of mixed lymphocyte culture and mitogen responsiveness. |
| |
Authors: | F DeLustro B F Argyris |
| |
Affiliation: | Laboratory of Molecular Genetics NICHD, NIH, Bethesda, Maryland 20014 USA |
| |
Abstract: | Murine spleen cells, stimulated in vitro by allogeneic spleen, display a strong proliferative response with the subsequent development of cytotoxic cells. This proliferation and sensitization can be abrogated by the addition of mitomycin-treated or X-irradiated murine DBA/2 mastocytoma cells (P-815). The substance required for this depression of lymphocyte responsiveness is present in the cell-free supernatant fluids of P-815 cultures. The suppression appears to be due to interference with cell proliferation in the mixed lymphocyte culture, because the P-815 also prevents spleen cells from proliferating in response to the mitogens concanavalin A (Con A), lipopolysaccharide (LPS), and phytohemagglutinin (PHA). The significance of these findings is discussed. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|