Heterogeneic modulation of malignant behavior in human glioma cells in defined and serum-containing media |
| |
Authors: | Hans K Haugland Ole-Bjørn Tysnes |
| |
Institution: | (1) The Gade Institute, Department of Pathology, University of Bergen, N-5021 Bergen, Norway;(2) Department of Neurology, University of Bergen, N-5021 Bergen, Norway |
| |
Abstract: | Summary Malignant features in three glioma cell lines were studied in four defined media of various complexity. The cell lines D37MG,
D54MG, and GaMG were able to grow in monolayer culture in all media examined, and as multicellular tumor spheroids in the
two most nutrient-rich media. In the defined media, none of the cell lines were able to migrate in a migration assay on poly-D-lysine-coated
plastic surfaces. Flow cytometric analysis of the GaMG cell line demonstrated no medium-dependent selection of subclones of
glioma cells in spheroids cultured for 30 d. Morphological diversity of spheroids varied according to the supplementation
of the media. The capacity of glioma cells to invade cellular rat brain aggregates was intact in the media examined. However,
glioma migration was severely inhibited by the lack of specific serum components. This study demonstrates that glioma growth
and invasion was heterogenously preserved in the defined media used. Depending on the assay to be used in the study of glioma
cell behavior, the degree of medium supplementation has to be considered. |
| |
Keywords: | glioma defined media monolayer spheroid proliferation migration invasion |
本文献已被 SpringerLink 等数据库收录! |
|