Dynamics of the spatial organization of the chromosome set in cells of <Emphasis Type="Italic">Drosophila melanogaster</Emphasis> imaginal disks normally and under the action of the tumor-inducing mutation <Emphasis Type="Italic">Merlin</Emphasis> |
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Authors: | L I Lebedeva E M Akhmametyeva L V Omelyanchuk |
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Institution: | 1.Institute of Cytology and Genetics,Russian Academy of Sciences,Novosibirsk,Russia |
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Abstract: | Fluorescence of H3-p histone and DAPI was studi ed at different stages of interphase and mitosis in cells of imaginal disks
of third-instar Drosophila melanogaster larvae. Three stages differing in the spatial organization of the chromosome set in mitosis were revealed. At the first stage
(prophase, prometaphase), the histone 3 phosphorylation level rises, and the volume occupied by the chromosome set in the
nucleus increases. The distinctive features of the second stage (metaphase) are a gradual decrease in the histone 3 phosphorylation
(the density of phosphorylation remaining constant) and a reduction of the volume occupied by the chromosome set. At the third
stage (anaphase, telophase), the intensity and density of the signal from H3-p histone decrease, and the volume occupied by
the chromosome set reduces. At this stage, in Mer
4 larvae, in contrast to the control strain, the cells prematurely pass from anaphase into telophase. In addition, a subpopulation
of cells with an abnormally large volume of nuclear DNA during the G1 period was revealed in Mer
4 larvae. The cells of this subpopulation do not enter into the DNA synthesis and quit the cycle. |
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