Cell wall polysaccharides from cell suspension cultures of the Atlantic Forest tree Rudgea jasminoides (Rubiaceae) |
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Authors: | Clóvis J F Oliveira Júnior Aline A Cavalari Nicholas C Carpita Marcos S Buckeridge Marcia R Braga |
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Institution: | 1. Núcleo de Pesquisa em Plantas Ornamentais, Instituto de Botanica, S?o Paulo, SP, Brazil 2. Universidade Federal de S?o Paulo, Campus Diadema, Diadema, SP, Brazil 3. Department of Plant Pathology, Purdue University, West Lafayette, IN, USA 4. Departamento de Botanica, Universidade de S?o Paulo, S?o Paulo, SP, Brazil 5. Núcleo de Pesquisa em Fisiologia e Bioquímica, Instituto de Botanica, C.P. 3005, S?o Paulo, SP, 01061-970, Brazil
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Abstract: | Rudgea jasminoides (Rubiaceae) is a tropical tree species native of the Atlantic Forest in the south of Brazil. Previous studies with leaf cell
walls of R. jasminoides showed a different proportion of cross-linked glycans compared to what is usually reported for eudicots. However, due to
the difficulties of working with whole plant organs, cell suspensions of R. jasminoides, consisting of predominantly undifferentiated cells with mainly primary cell walls, were used to examine cell walls and extracellular
soluble polysaccharides (EP) released into the culture medium. Sugar composition and linkage analysis showed homogalacturonans,
xylogalacturonans and arabinogalactans to be the predominant EP. In the cell wall, homogalacturonans and arabinogalactans
are the major pectins, and xyloglucans and xylans are the major cross-linking glycans. The presence of xylogalacturonans in
the R. jasminoides cell cultures seems to be related to the occurrence of a homogeneous cell suspension with loosely attached cells. Although
all alkali extractions from the cell walls yielded amounts of xyloglucan that exceed those of the xylans, the latter was found
in a proportion that is higher than what has been usually reported for primary cell walls of most eudicots. The xyloglucan
from cell walls of cell suspension cultures of R. jasminoides has low fucosylation levels and high proportion of galactosyl residues, a branching pattern commonly found in storage cell-wall
xyloglucans. |
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