Genotype-dependent differences in S12-RNase expression lead to sporadic self-compatibility |
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Authors: | Qin X. Luu D.T. Yang Q. Maës O. Matton D.P. Morse D. Cappadocia M. |
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Affiliation: | (1) Département de Sciences Biologiques, Canada;(2) Institut de Recherche en Biologie Végétale, Université de Montréal, 4101 Sherbrooke est, Montréal, Québec, Canada , H1X 2B2 |
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Abstract: | Sporadic self-compatibility, the occasional fruit formation after otherwise incompatible pollinations, has been observed in some S12-containing genotypes of Solanum chacoense but not in others. We have sequenced this S12 allele and analyzed its expression in four different genotypes. The S12-RNase levels were generally less abundant than those of other S-RNases present in the same plants. In addition, two-fold and five-fold differences in the amount of S12-RNase and S12 RNA, respectively, were observed among the genotypes analyzed. A comparison with the genetic data showed that genotypes with the highest levels were fully and permanently self-incompatible, whereas those with the lowest levels were those in which sporadic self-compatibility had been observed. The mature protein contains four potential glycosylation sites and genotype-specific differences in the pattern of glycosylation are also observed. Our results suggest the presence of modifier genes which affect, in a genotype-dependent manner, the level of expression and the post-translational modification of the S12-RNase. |
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Keywords: | glycosylation modifier genes self-compatibility Solanum chacoense S-RNase |
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