An enzymatic-isotopic microassay for putrescine |
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| Authors: | Sami I. Harik Gavril W. Pasternak Solomon H. Snyder |
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| Affiliation: | Departments of Pharmacology and Experimental Therapeutics and Psychiatry and the Behavioral Sciences, The Johns Hopkins University School of Medicine, Baltimore, Md. 21205 U.S.A. |
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| Abstract: | A highly sensitive enzymatic isotopic microassay procedure for the measurement of putrescine (1,4-diaminobutane) is described. The method depends on the enhancement by putrescine of the decarboxylation of S-adenosyl-L-[carboxy-14C] methionine by baker's yeast (Saccharomyces cerevisiae) S-adenosyl-L-methionine decarboxylase in the presence of varying amounts of putrescine. The quantity of 14CO2 evolved is a linear function of the amount of putrescine present. This method was used to measure the putrescine content of various tissues. |
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