Comparison of the Stereospecificity and Immunoreactivity of NADH-Ferricyanide Reductases in Plant Membranes |
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| Authors: | Fredlund K M Struglics A Widell S Askerlund P Kader J C Moller I M |
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| Institution: | Department of Plant Biology (K.M.F., A.S., S.W., I.M.M.) and Department of Plant Biochemistry (P.A.), Lund University, Box 117, S-221 00 Lund, Sweden. |
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| Abstract: | The substrate stereospecificity of NADH-ferricyanide reductase activities in the inner mitochondrial membrane and peroxisomal membrane of potato (Solanum tuberosum L.) tubers, spinach (Spinacea oleracea L.) leaf plasma membrane, and red beetroot (Beta vulgaris L.) tonoplast were all specific for the beta]-hydrogen of NADH, whereas the reductases in wheat root (Triticum aestivum L.) endoplasmic reticulum and potato tuber outer mitochondrial membrane were both alpha]-hydrogen specific. In all isolated membrane fractions one or several polypeptides with an apparent size of 45 to 55 kD cross-reacted with antibodies raised against a microsomal NADH-ferricyanide reductase on western blots. |
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