Biosynthesis of active Bacillus subtilis urease in the absence of known urease accessory proteins |
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Authors: | Kim Jong Kyong Mulrooney Scott B Hausinger Robert P |
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Institution: | Cell and Molecular Biology Program, Michigan State University, East Lansing, Michigan 48824-4320, USA. |
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Abstract: | Bacillus subtilis contains urease structural genes but lacks the accessory genes typically required for GTP-dependent incorporation of nickel. Nevertheless, B. subtilis was shown to possess a functional urease, and the recombinant enzyme conferred low levels of nickel-dependent activity to Escherichia coli. Additional investigations of the system lead to the suggestion that B. subtilis may use unidentified accessory proteins for in vivo urease activation. |
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