Enzymatic catalysis in a supercritical fluid |
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Authors: | T. W. Randolph H. W. Blanch J. M. Prausnitz C. R. Wilke |
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Affiliation: | (1) Department of Chemical Engineering College of Chemistry, University of California, 94720 Berkeley, California, USA |
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Abstract: | The enzyme alkaline phosphatase, EC 3. 1. 3. 1, was found to be active in a supercritical carbo dioxide solvent system. A batch reaction of disodium p-nitrophenyl phosphate with a 0.1 vol. % water solution in supercritical CO2 at 100 atm and 35°C produced p-nitrophenol when catalyzed by alkaline phosphatase. |
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