Enhanced lignin peroxidase synthesis by Phanerochaete Chrysosporium in solid state bioprocessing of a lignocellulosic substrate |
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| Authors: | M. Asgher M.J. Asad R.L. Legge |
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| Affiliation: | (1) Department of Chemistry, University of Agriculture, Faisalabad, Pakistan;(2) Department of Chemical Engineering, University of Waterloo, Ontario, Canada |
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| Abstract: | Summary A solid state fermentation (SSF) process for the production of lignin peroxidase was optimized to enhance enzyme production by Phanerochaete chrysosporium. Optimization of the corncob SSF medium caused a significant reduction in fermentation time to give maximum lignin peroxidase yield. Supplementation of the SSF medium by low concentrations of peptone, yeast extract and Tween-80 enhanced lignin peroxidase production. Maximum yield of lignin peroxidase was 13.7 U/gds (units per gram dry substrate) noted after 5 days of SSF with 70% moisture and 20% (v/w) inoculum. |
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| Keywords: | Enhanced production Phanerochaete chrysosporium Lignin peroxidase Optimization Solid state fermentation |
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