Flow Cytometric Determination of Genome Size in some Old World Lupinus Species (Fabaceae)

Abstract: Genome size determinations using propidium iodide flow cytometry were conducted in Lupinus anatolicus, L. pilosus, L. luteus, L. hispanicus ssp. hispanicus and L. hispanicus ssp. hispa-nicus × L. luteus (a complex hybridization product ofL. hispanicus ssp. hispanicus and L. luteus ). Using Glycine max "Ceresia" (1C = 1.134pg) and an accession of L. pilosus (1C = 0.649pg) as internal standards the following 1C values were obtained: L. anatolicus: 0.606 pg; L. pilosus: 0.620–0.678 pg; L. luteus: 1.173 pg; L. hispanicus: 1.043 pg; L. hispanicus ssp. hispanicus × L luteus: 1.036 pg. Lupinus anatolicus is a newly described taxon with smooth seeds whose genome is approximately as small as the smallest genomes measured in the related L. pilosus , a taxon with rough seeds. The genomes in the related smooth-seeded taxa L. luteus, L. hispanicus and L. hispanicus ssp. hispanicus × L. luteus are considerably larger. There is a 1.13-fold difference between L. luteus (crop) and L. hispanicus (wild). The genome size of L. hispanicus ssp. hispanicus × L. luteus , however, is not intermediate, it is close to L. hispanicus .