New microsatellite markers in chicken optimized for automated fluorescent genotyping |
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| Authors: | R P M A Crooijmans R J M Dijkhof J J van der Poel & M A M Groenen |
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| Institution: | Wageningen Institute of Animal Sciences, Department of Animal Breeding, Wageningen Agricultural University, PO Box 338, 6700 AH Wageningen, The Netherlands |
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| Abstract: | We have isolated and developed 180 new polymorphic chicken microsatellite markers. In addition, primers have been developed for 91 microsatellites derived from the GenBank sequence database (isolated by the laboratory of Terry Burke, Leicester University), of which 89 were polymorphic, and six existing polymorphic markers (HUJ) have been modified. The primer sequences were designed to allow optimal performance of the markers, in sets containing multiple microsatellites, on ABI sequencers. The average number of alleles for the 275 polymorphic markers described was 4·0. Of these markers, 93% were polymorphic in the Wageningen resource population whereas 57% of the markers were polymorphic in the East Lansing reference population and only 44% could be mapped in the Compton reference population. The microsatellite markers described in this paper, in combination with the microsatellite markers published previously, are particularly well suited for performing a total genome scan for the detection of quantitative trait loci (QTL). |
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| Keywords: | chicken microsatellite |
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