STIMULATORY EFFECTS OF SUBSTANCE P ON NEURITE EXTENSION AND CYCLIC AMP LEVELS IN CULTURED NEUROBLASTOMA CELLS |
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Authors: | Shigehiko Narumi Yoshitaka Maki |
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Institution: | Biological Research Laboratories, Central Research Divisions Takeda Chemical Industries, Ltd., Yodogawa-ku, Osaka, Japan |
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Abstract: | Abstract— Synthetic substance P initially increased cyclic AMP levels and subsequently induced neurite extension in cultured neuroblastoma N 18 cells. The magnitude of these effects depended on the concentration of fetal calf serum (FCS) in the culture medium, being more evident in the presence of a lower (0.1%) concentration of FCS. In Eagle's medium containing 0.1% FCS, low concentrations of substance P (10?7-10?5 M) increased cyclic AMP levels and stimulated neurite extension. In Eagle's medium containing 5%FCS, both substance P at concentrations of 10?5-10?3M and dibutyryl cyclic AMP at concentrations of 10?4-10?2M increased cyclic AMP levels and stimulated neurite extension. The activities of acetylcholinesterase, (Na++ K+)-, HCO3? and Mg2+ -stimulated-ATPase were also increased. Cell growth was inhibited. Substance P at concentrations of 10-7-10?5M also stimulated the adenylate cyclase activity of a particulate fraction of N 18 in a concentration-dependent manner. |
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