Characterization of interleukin-1 receptor-associated kinase 1 binding protein 1 gene in small abalone Haliotis diversicolor |
| |
Authors: | Hui Ge Guodong Wang Lili Zhang Shuhong Wang Zhihua Zou Sufen Yan Yilei Wang Ziping Zhang |
| |
Institution: | Key Laboratory of Healthy Mariculture in the East China Sea, Ministry of Agriculture, Fisheries College, Jimei University, Xiamen, 361021, China; Fisheries Research Institute of Fujian, Xiamen, 361012 China. |
| |
Abstract: | Interleukin receptor-associated kinase (IRAK)-1 binding protein 1 (IRAK1BP1) is a critical factor in preventing dangerous overproduction of proinflammatory cytokines by the innate immune system and in influencing the specificity of TLR responses. In this study, a first molluscan IRAK1BP1 gene, saIRAK1BP1, was cloned from the small abalone (Haliotis diversicolor). Its full-length cDNA sequence is 1047bp, with a 747bp open reading frame encoding a protein of 249 aa. The molecular mass of the deduced protein is approximately 28.1kDa with an estimated pI of 8.87, and shows highest identity (52%) to acorn worm Saccoglossus kowalevskii. Amino acid sequence analysis revealed that saIRAK1BP1 shares a conserved SIMPL domain. Quantitative real-time PCR was employed to investigate the tissue distribution of saIRAK1BP1 mRNA, and its expression in abalone under bacteria challenge and larvae at different developmental stages. The saIRAK1BP1 mRNA could be detected in all examined tissues, with the highest expression level in hemocytes, and was up-regulated in gills, kidneys and hemocytes after bacteria injection. Additionally, saIRAK1BP1 was constitutively expressed at all examined developmental stages. These results indicate that saIRAK1BP1 play an important role in the adult abalone immune system and might be essential in embryo and larval development in abalone. |
| |
Keywords: | IRAK1BP1 interleukin receptor‐associated kinase 1 binding protein 1 TLRs Toll-like receptors IRAK IL-1 receptor‐associated kinase IRAK-M IL-1 receptor‐associated kinase-M IL-12 interleukin-12 MyD88s myeloid differentiation factor 88s SOCS-1 Suppressor of cytokine signaling-1 Triad3A zinc finger protein inhibiting NF-κB Trim Tripartite-motif protein A20 TNF-α-induced protein 3 UBA ubiquitinating enzyme A CYLD cylindromatosis protein SARM Sterile alpha and Armadillo motif containing protein IRF-4 interferon regulatory factor 4 PI3K phosphoinositide 3-kinase SIMPL pelle-like kinase TBT tributyltin LTA lipoteichoic acid PBMCs peripheral blood mononuclear cells |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|