Initial cleavage site of the oxidized insulin B-chain by a ribosomal proteinase fromStreptococcus lactis,a food microorganism |
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Authors: | Dr Eiji Ichishima Yasuhiro Kawai Michio Takeuchi Kenkichi Ahiko |
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Institution: | (1) Laboratory of Enzymology and Microbial Chemistry, Tokyo University of Agriculture and Technology, Fuchu, 183 Tokyo, Japan;(2) Technical Laboratories of Snow Brand Products Company, Kawagoe, Saitama, Japan |
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Abstract: | The unique specificity of a ribosomal proteinase fromStreptococcus lactis, a food microorganism, was investgated with the oxidized insulin B-chain. The initial cleavage site of the enzyme was observed at Leu15-Tyr16 bond, and additional site at Arg22-Gly23 |
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