Genotyping of archival Atlantic salmon scales from northern Quebec and West Greenland using novel PCR primers for degraded mtDNA |
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| Authors: | D Knox K Lehmann D G Reddin E Verspoor |
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| Institution: | FRS Marine Laboratory, P.O. Box 101, Aberdeen AB11 9DB, Scotland, U.K.;Ministry of Food, Agriculture and Fisheries, Directorate for Food, Fisheries and Agro Business, Kampmannsgade 3, DK-1780 Copenhagen V, Denmark;Department of Fisheries and Oceans, P.O. Box 5667, St. John's, Newfoundland, Canada A1C5X1 |
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| Abstract: | PCR primers were successfully designed to amplify small ND1 gene fragments for RFLP genotyping of degraded Atlantic salmon Salmo salar mtDNA. Analysis of archival scales with these primers, when existing primer sets failed, show Atlantic salmon from the George River, Quebec, to include European haplotypes and those from the Kapisidlit River, West Greenland, to be fixed for a European haplotype characteristic of Baltic populations. |
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| Keywords: | salmonids PCR primers archival mtDNA phylogeography |
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