Establishment of callus and cell suspension cultures of raspberry (Rubus idaeus cv. Royalty)

Callus cultures were initiated from leaf sections of raspberry (Rubus idaeus L.) cv. Royalty. Explants from younger leaves produced significantly more calli than those from older leaves. Anderson's salt mixture was more efficient for callus induction than the Murashige-Skoog medium. The best propagation and growth of calli was observed on Anderson's medium supplemented with 9 M 2,4-dichlorophenoxyacetic acid, 4.9 M indolebutyric acid and 4.9 M 6-(dimethylallylamino)-purine. During a 28-day period, the fresh weight of calli increased approximately five times. The same medium without agar was used for establishing cell suspension cultures. Fresh weight of cells increased four times and dry weight approximately doubled during 10 days of culture.Abbreviations 2,4-d 2,4-dichlorophenoxyacetic acid - 2iP 6-(dimethylallylamino)-purine - IBA indolebutyric acid - MS Murashige & Skoog basal salt medium