The composition of cell wall skeleton and outermost lipids of Mycobacterium vaccae is modified by ethambutol treatment |
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Authors: | Anna Rumijowska-Galewicz Ma?gorzata Korycka-Macha?a Katarzyna Lisowska Jaros?aw Dziadek |
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Institution: | Institute for Medical Biology Polish Academy of Sciences, ?ód?, Poland. arumijowska@cbm.pan.pl |
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Abstract: | Ethambutol (EMB) is a first line drug in tuberculosis treatment inhibiting the biosynthesis of arabinogalactan, which is a component of the mycobacterial cell wall. The growth of Mycobacterium vaccae cells in the presence of EMB increases cell wall permeability, which was monitored by beta-sitosterol biotransformation. GC/MS and GLC/MS (gas chromatography/mass spectrometry) analysis revealed dramatic changes in the content of covalently bound mycolic acids and in molar ratio galactose (Gal) to arabinose (Ara) in the cell envelopes of EMB-treated cells. The detected variations in the compositions of fatty acids indicate that both the cell wall skeleton and outer layer (free lipids) are decomposed due to EMB treatment. |
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