The eutD gene of Salmonella enterica encodes a protein with phosphotransacetylase enzyme activity |
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| Authors: | Brinsmade Shaun R Escalante-Semerena Jorge C |
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| Institution: | Department of Bacteriology, University of Wisconsin-Madison, Madison, Wisconsin 53726, USA. |
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| Abstract: | The EutD protein of Salmonella enterica is homologous to the catalytic domain of the phosphotransacetylase (Pta) enzyme. The Pta-like activity level of the EutD enzyme compared favorably to that of other Pta enzymes. High-pressure liquid chromatography and mass spectrometry verified that acetyl-coenzyme A was the product of the reaction. The EutD protein restored growth of an S. enterica pta strain on acetate as the source of carbon and energy. |
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