Demonstration and characterization of angiotensin-converting enzyme in human pituitary tissue |
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| Authors: | M Tani K Mizuno S Hashimoto N Kunii S Niimura R Yabe H Watari S Fukuchi |
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| Affiliation: | 1. INSERM Unit 1088, Jules Verne University of Picardie, Amiens, France;2. Division of Nephrology, Ambroise Paré University Hospital, AP-HP, Paris-Ile-de-France-Ouest University (UVSQ), Boulogne-Billancourt, France;1. Service d''Oncologie Médicale, Institut National d’Oncologie, CHU Rabat and Université Mohamed V, Rabat, Morocco;2. Institut de Cancérologie, Hospices Civils de Lyon and Université de Lyon, Pierre-Bénite Cedex, France;1. Internal Medicine, Ohio State University, Columbus, OH;;2. Medicine, University of Colorado, Aurora, CO; and;3. Department of Pathology, Ohio State University, Columbus, OH;1. Laboratory of Experimental Nephrology, Faculty of Medicine, Université Libre de Bruxelles (ULB), Brussels, Belgium;2. Laboratory of Pharmacognosy, Bromatology and Human Nutrition, Faculty of Pharmacy, Université Libre de Bruxelles (ULB), Brussels, Belgium;3. Laboratory of Physiology and Ethnopharmacology, URAC-40, Department of Biology, Faculty of Sciences, Mohammed First University, Oujda, Morocco;4. Laboratory of Therapeutical Chemistry and Pharmacognosy, Université de Mons (UMONS), Mons, Belgium |
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| Abstract: | High activity of angiotensin-converting enzyme was demonstrated in human pituitary tissue. This activity required the presence of chloride ion and was almost completely inhibited by a specific converting enzyme inhibitor captopril (10 nM), indicating that the activity measured is indeed angiotensin-converting enzyme. The specific activity of the enzyme was 1.68 +/- 1.20 nmol hippuric acid generated mg of protein-1 min-1 (mean +/- SD, for 11 specimens). The biochemical features of the enzyme were closely related to the well-characterized human lung converting enzyme, such as molecular weight (290,000), optimum pH (8.0-8.5), the presence of glycoprotein residues, and dependence on chloride ion concentration. These results provide definitive evidence for the presence of angiotensin-converting enzyme in human pituitary tissue. |
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