Induction of tolerance to oxidative stress in the green alga, Chlamydomonas reinhardtii, by abscisic acid |
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Authors: | K YOSHIDA E IGARASHI M MUKAI K HIRATA & K MIYAMOTO |
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Institution: | Environmental Bioengineering Laboratory, Graduate School of Pharmaceutical Sciences, Osaka University, 1–6 Yamada-oka, Suita, Osaka 565–0871, Japan |
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Abstract: | The effect of abscisic acid (ABA) on the tolerance to oxidative stress in a freshwater green alga, Chlamydomonas reinhardtii, was investigated. Exogenously added ABA enhanced the growth of this alga, which was observed under continuous illumination but not in the dark. The cells treated with ABA for 24 h showed tolerance to oxidative stress caused by exposure to paraquat or hydrogen peroxide. In the ABA‐treated cells, the activities of two antioxidant enzymes, catalase (CAT) and ascorbate peroxidase (APX), were significantly higher than those in the untreated control. The result suggests that ABA plays a role in the enhancement of tolerance to oxidative stress by increasing the activity of antioxidant enzymes. |
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Keywords: | ascorbate peroxidase catalase 2' 7'-dichlorofluorescein diacetate (DCFH-DA) flow cytometry hydrogen peroxide paraquat plant hormone reactive oxygen species |
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