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Induction of tolerance to oxidative stress in the green alga, Chlamydomonas reinhardtii, by abscisic acid
Authors:K YOSHIDA  E IGARASHI  M MUKAI  K HIRATA  & K MIYAMOTO
Institution:Environmental Bioengineering Laboratory, Graduate School of Pharmaceutical Sciences, Osaka University, 1–6 Yamada-oka, Suita, Osaka 565–0871, Japan
Abstract:The effect of abscisic acid (ABA) on the tolerance to oxidative stress in a freshwater green alga, Chlamydomonas reinhardtii, was investigated. Exogenously added ABA enhanced the growth of this alga, which was observed under continuous illumination but not in the dark. The cells treated with ABA for 24 h showed tolerance to oxidative stress caused by exposure to paraquat or hydrogen peroxide. In the ABA‐treated cells, the activities of two antioxidant enzymes, catalase (CAT) and ascorbate peroxidase (APX), were significantly higher than those in the untreated control. The result suggests that ABA plays a role in the enhancement of tolerance to oxidative stress by increasing the activity of antioxidant enzymes.
Keywords:ascorbate peroxidase  catalase  2'  7'-dichlorofluorescein diacetate (DCFH-DA)  flow cytometry  hydrogen peroxide  paraquat  plant hormone  reactive oxygen species
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