Efficient generation of antigen-specific cytotoxic T cells using retrovirally transduced CD40-activated B cells |
| |
Authors: | Kondo Eisei Topp Max S Kiem Hans-Peter Obata Yuichi Morishima Yasuo Kuzushima Kiyotaka Tanimoto Mitsune Harada Mine Takahashi Toshitada Akatsuka Yoshiki |
| |
Affiliation: | Division of Immunology, Aichi Cancer Center Research Institute, Nagoya, Japan. |
| |
Abstract: | The development of rapid, efficient, and safe methods for generating Ag-specific T cells is necessary for the clinical application of adoptive immunotherapy. We show that B cells stimulated with CD40 ligand and IL-4 (CD40-B cells) can be efficiently transduced with retroviral vectors encoding a model Ag, CMV tegument protein pp65 gene, and maintain high levels of costimulatory molecules after gene transfer. CTL lines specific for pp65 were readily generated in all four healthy CMV-seropositive donors by stimulating autologous CD8(+) T cells with these transduced CD40-B cells, both of which were derived from 10 ml peripheral blood. ELISPOT assays revealed that the CTL lines used multiple HLA alleles as restricting elements. Thus, CD40-B cells transduced retrovirally with Ag-encoding cDNA can be potent APC and facilitate to generate Ag-specific CTL in vitro. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|