Transformation of Zymomonas mobilis by a hybrid plasmid |
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| Authors: | G M Browne M L Skotnicki A E Goodman P L Rogers |
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| Institution: | 1. School of Biotechnology, University of New South Wales, Kensington 2033, Australia;1. Virus Ecology Research Group, Research School of Biological Sciences, Australian National University, ACT 2601, Australia |
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| Abstract: | The transformation of Zymomonas mobilis by plasmid DNA was achieved using a modification of the CaCl2 method for Escherichia coli. The highest frequency of transformation obtained was 5 × 103 transformants/μg DNA. The success of the method depended upon the use of a plasmid which is a cointegrate between a Z. mobilis cryptic plasmid and an E. coli plasmid carrying two selectable drug resistance markers. |
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