Control of sporulation in yeast: SAD1-a mating-type specific, unstable alteration that uncouples sporulation from mating-type control |
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Authors: | Anita K Hopper and Vivian L MacKay |
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Institution: | (1) Department of Biological Chemistry, The Milton S. Hershey Medical Center, The pennsylvania State University, 17033 Hershey, Pennsylvania, USA;(2) Walsman Institute of Microbiology, Rutgers University, P.O. Box 759, 08854 Piscataway, New Jersey, USA |
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Abstract: | Summary Normally, in order to sporulate, a diploid yeast cell must be heterozygous (MAT
a/MAT ) at the mating-type locus. In a new mutant, this requirement is circumvented by SAD1. This alteration is mating-type specific; it allows sporulation of MAT / MAT . MAT /mat
a-1, and MAT /mat -2 diploid cells, but not MAT
a/MAT
a or MAT
a/0 (monosomic) strains. Other than acquiring the ability to sporulate, SAD1 cells behave as wild-type MAT /MAT strains; they exhibit medial budding, normal mating factor production and response and mate with normal mating efficiencies and kinetics. The segregation of SAD1 is often bizarre; for example, MAT /MAT strains which were constructed to be heterozygous SAD1/+ often segregate 4 SAD1:0+progeny and strains which were constructed to be homozygous SAD1/ SAD1 sometimes segregated 1 SAD1:3+progeny. The genetic analyses of SAD1 suggest that it is dominant and is located 30 cM from MAT on chromosome III. |
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