Initiation points for DNA replication in nontransformed and simian virus 40-transformed Chinese hamster lung cells. |
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Authors: | R G Martin A Oppenheim |
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Institution: | Laboratory of Molecular Biology National Institute of Arthritis, Metabolism and Digestive Diseases Bethesda, Maryland 20014 USA |
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Abstract: | Randomly growing Chinese hamster lung cells were pulse-labeled with 3H-thymidine, and the replicating forks of individual DNA fibers were visualized by autoradiography. When grown in complete medium, wild-type SV40-transformed cells had more forks per unit length of DNA than nontransformed cells. In isoleucine-depleted medium, wild-type SV40-transformed cells had fewer forks per unit length than those few nontransformed cells (1-3% of the population) which continued DNA replication. Cells transformed by a tsA mutant of SV40 when grown at the permissive temperature had more forks per unit length in complete medium and fewer forks per unit length in depleted medium than nontransformed cells, but when grown at the restrictive temperature, the tsA-transformed cells behaved like nontransformed cells. |
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