Effect of stress on the ability of a phlA-based quantitative competitive PCR assay to monitor biocontrol strain Pseudomonas fluorescens CHA0 |
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| Authors: | Rezzonico Fabio Moënne-Loccoz Yvan Défago Geneviève |
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| Institution: | Phytopathology Group, Institute of Plant Science, Swiss Federal Institute of Technology (ETH), CH-8092 Zürich, Switzerland. |
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| Abstract: | A quantitative competitive PCR (QC-PCR) assay targeting the phlA gene of Pseudomonas fluorescens CHA0 was developed and tested in vitro. Statistically significant, positive correlations were found between QC-PCR and both CFU and total cell number when studying cells in log or stationary phase. The correlations disappeared when considering stressed cells. |
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