Photo-oxidation of tyrosine in a bio-engineered bacterioferritin ‘reaction centre’—A protein model for artificial photosynthesis |
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Authors: | Kastoori Hingorani Ron Pace Spencer Whitney James W Murray Paul Smith Mun Hon Cheah Tom Wydrzynski Warwick Hillier |
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Institution: | 1. Building 134, Linnaeus Way, Research School of Biology, The Australian National University, ACT 0200, Australia;2. Building 137, Sullivans Creek Road, Research School of Chemistry, The Australian National University, ACT 0200, Australia;3. 724 Sir Ernst Chain Building, South Kensington Campus, Division of Molecular Biosciences, Imperial College London, London SW7 2AZ, United Kingdom |
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Abstract: | The photosynthetic reaction centre (RC) is central to the conversion of solar energy into chemical energy and is a model for bio-mimetic engineering approaches to this end. We describe bio-engineering of a Photosystem II (PSII) RC inspired peptide model, building on our earlier studies. A non-photosynthetic haem containing bacterioferritin (BFR) from Escherichia coli that expresses as a homodimer was used as a protein scaffold, incorporating redox-active cofactors mimicking those of PSII. Desirable properties include: a di-nuclear metal binding site which provides ligands for bivalent metals, a hydrophobic pocket at the dimer interface which can bind a photosensitive porphyrin and presence of tyrosine residues proximal to the bound cofactors, which can be utilised as efficient electron-tunnelling intermediates. |
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Keywords: | Artificial photosynthesis Protein engineering Electron transfer Tyrosine oxidation Photosystem II Bacterioferritin |
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