CD8CD25 T cells reduce atherosclerosis in apoE(−/−) mice |
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Authors: | Jianchang Zhou Paul C. Dimayuga Xiaoning Zhao Juliana YanoWai Man Lio Portia TrinidadTomoyuki Honjo Bojan CercekPrediman K. Shah Kuang-Yuh Chyu |
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Affiliation: | Oppenheimer Atherosclerosis Research Center, Division of Cardiology, Cedars-Sinai Heart Institute, Cedars-Sinai Medical Center, Los Angeles, CA 90048, United States |
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Abstract: | BackgroundIt is increasingly evident that CD8+ T cells are involved in atherosclerosis but the specific subtypes have yet to be defined. CD8+CD25+ T cells exert suppressive effects on immune signaling and modulate experimental autoimmune disorders but their role in atherosclerosis remains to be determined. The phenotype and functional role of CD8+CD25+ T cells in experimental atherosclerosis were investigated in this study.Methods and resultsCD8+CD25+ T cells were observed in atherosclerotic plaques of apoE(−/−) mice fed hypercholesterolemic diet. Characterization by flow cytometric analysis and functional evaluation using a CFSE-based proliferation assays revealed a suppressive phenotype and function of splenic CD8+CD25+ T cells from apoE(−/−) mice. Depletion of CD8+CD25+ from total CD8+ T cells rendered higher cytolytic activity of the remaining CD8+CD25− T cells. Adoptive transfer of CD8+CD25+ T cells into apoE(−/−) mice suppressed the proliferation of splenic CD4+ T cells and significantly reduced atherosclerosis in recipient mice.ConclusionsOur study has identified an athero-protective role for CD8+CD25+ T cells in experimental atherosclerosis. |
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Keywords: | Atherosclerosis CD8+CD25+ T cells Immune modulation Adoptive transfer |
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