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The ultrastructure of Chlorobaculum tepidum revealed by cryo-electron tomography
Authors:Misha Kudryashev  Aikaterini Aktoudianaki  Dimitrios Dedoglou  Henning Stahlberg  Georgios Tsiotis
Institution:1. Center for Cellular Imaging and NanoAnalytics (C-CINA), Biozentrum, University of Basel, 4058 Basel, Switzerland;2. Focal Area Infection Biology, Biozentrum, University of Basel, 4058 Basel, Switzerland;3. Division of Biochemistry, Department of Chemistry, University of Crete, 71003 Voutes, Heraklion, Greece
Abstract:Chlorobaculum (Cba) tepidum is a green sulfur bacterium that oxidizes sulfide, elemental sulfur, and thiosulfate for photosynthetic growth. As other anoxygenic green photosynthetic bacteria, Cba tepidum synthesizes bacteriochlorophylls for the assembly of a large light-harvesting antenna structure, the chlorosome. Chlorosomes are sac-like structures that are connected to the reaction centers in the cytoplasmic membrane through the BChl α-containing Fenna–Matthews–Olson protein. Most components of the photosynthetic machinery are known on a biophysical level, however, the structural integration of light harvesting with charge separation is still not fully understood. Despite over two decades of research, gaps in our understanding of cellular architecture exist. Here we present an in-depth analysis of the cellular architecture of the thermophilic photosynthetic green sulfur bacterium of Cba tepidum by cryo-electron tomography. We examined whole hydrated cells grown under different electron donor conditions. Our results reveal the distribution of chlorosomes in 3D in an unperturbed cell, connecting elements between chlorosomes and the cytoplasmic membrane and the distribution of reaction centers in the cytoplasmic membrane.
Keywords:AFM  atomic force microscopy  3D  three dimensional  BChl  bacteriochlorophyll  Cba  Chlorobaculum  CM  cytoplasmic membrane  Cryo-ET  cryo-electron tomography  DLS  dynamic light scattering  EM  electron microscopy  FMO  Fenna&ndash  Mathew&ndash  Olson  GSB  green sulfur bacteria  OM  outer membrane  PFT-AFM  peak force tapping atomic force microscopy  PG  peptidoglycan  RC  reaction center  TEM  transmission electron microscopy
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