Measuring localization and diffusion coefficients of basolateral proteins in lateral versus basal membranes using functionalized substrates and kICS analysis |
| |
| Authors: | Saw Marlar Eva C ArnspangJennifer S Koffman Lene N Nejsum |
| |
| Institution: | Institute of Molecular Biology and Genetics and Interdisciplinary Nanoscience Center, Aarhus University, C. F. Moellers Allé 3, Aarhus, Denmark |
| |
| Abstract: | Micropatterning enabled semiquantitation of basolateral proteins in lateral and basal membranes of the same cell. Lateral diffusion coefficients of basolateral aquaporin-3 (AQP3-EGFP) and EGFP-AQP4 were extracted from “lateral” and “basal” membranes using identical live-cell imaging and k-space Image Correlation Spectroscopy (kICS). |
| |
| Keywords: | (4-6) Diffusion coefficient kICS: k-space Image Correlation Spectroscopy Aquaporin MDCK Micropatterning |
| 本文献已被 ScienceDirect 等数据库收录！ |
